THE FINE STRUCTURE OF BOVINE NASAL CARTILAGE
نویسندگان
چکیده
منابع مشابه
The Fine Structure of Bovine Nasal Cartilage
Bovine nasal cartilage was studied by electron microscopy before and after extraction with 4 M guanidinium chloride or 1.9 M CaCl(2). These solvents removed matrix granules, basophilia, and 85% of the proteoglycan complex, measured as hexuronate. Simultaneously, many collagen fibrils were disaggregated into component microfibrils (approximately 40 A thick). In contrast to the above solvents, ex...
متن کاملTHE FINE STRUCTURE OF BOVINE NASAL CARTILAGE Extraction as a Technique to Study Proteoglycans and Collagen in Cartilage Matrix
Bovine nasal cartilage was studied by electron microscopy before and after extraction with 4 M guanidinium chloride or 1 .9 M CaC12. These solvents removed matrix granules, basophilia, and 85% of the proteoglycan complex, measured as hexuronate . Simultaneously, many collagen fibrils were disaggregated into component microfibrils (approximately 40 A thick) . In contrast to the above solvents, e...
متن کاملProteinase inhibitors of bovine nasal cartilage.
Extracts from bovine nasal cartilage with 1 M-guanidinium chloride were fractionated by ultrafiltration. Gel chromatography of the low-molecular-weight material resolved three distinct fractions with inhibitory activity against (a) collagenases (22000 mol.wt.), (b) thiol proteinases cathepsin B and papain (13000 mol.wt.), and (c) trypsin and other serine proteinases (7000 mol.wt.).
متن کاملStudies on the Properties of the Bovine Nasal Cartilage
Bovine nasal cartilage was repeatedly extracted with the dissociative solvent, 4 M guanidinium chloride. About 25% of the total tissue proteoglycans could not be solubilized as judged by galactosamine analysis. The inextractable proteoglycan could be at least partially solubilized by treating the guanidine-extracted tissue with reagents which completely or partially degraded the proteoglycan pr...
متن کاملCharacteristics of the nonaggregating proteoglycans isolated from bovine nasal cartilage.
Purified proteoglycans from bovine nasal cartilage (the Al fraction) were separated by sedimentation velocity centrifugation into a fraction enriched in aggregates (Al-Sl) and a fraction of nonaggregated proteoglycans (Al-S2), containing about 85% and 15% of the starting material, respectively. Both fractions were centrifuged in a dissociative CsCl density gradient to remove non-proteoglycan pr...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1971
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.49.3.650